Thymosin Alpha-1 (5 mg Vial) Dosage Protocol
Research guide for reconstitution and dosing of Thymosin Alpha-1 (Tα1), a 28–amino-acid immunomodulatory peptide investigated for immune regulation, T-cell maturation, and host-defense support.
Quickstart Highlights
Thymosin Alpha-1 (Tα1) is a 28–amino-acid peptide derived from prothymosin alpha and originally isolated from the thymus gland. Research characterizes it as an immunomodulator that helps regulate T-cell maturation, dendritic-cell function, and innate immune signaling (including Toll-like receptor pathways), supporting a balanced rather than broadly stimulated or suppressed immune response. Important note: Thymosin Alpha-1 is not FDA-approved for human use in the United States and is for research purposes only.
- Reconstitute: Add 1.0 mL bacteriostatic water → approximately 5 mg/mL concentration
- Typical research dosing: 1600 mcg (1.6 mg) twice weekly is the most commonly referenced protocol
- Easy measuring: At 5 mg/mL, 1 insulin unit ≈ 50 mcg on a U-100 syringe (1600 mcg ≈ 32 units)
- Storage: Refrigerate at 2–8 °C (35.6–46.4 °F), protected from light
Dosing & Reconstitution Guide
Educational subcutaneous protocol based on commonly referenced twice-weekly dosing
Subcutaneous Protocol (5 mg vial, 1 mL = 5 mg/mL)
| Protocol | Dose | Units (per injection) | Measurement (mL) |
|---|---|---|---|
| Standard maintenance | 1600 mcg twice weekly | 32 units | 0.32 mL |
| Intensive / acute support | 1600 mcg once daily (5–7 days) | 32 units | 0.32 mL |
| Lower-dose protocol | 800 mcg twice weekly | 16 units | 0.16 mL |
Administration: Subcutaneous injection, most commonly twice weekly. Some research protocols use a short daily course during acute immune challenges, then transition to a twice-weekly maintenance schedule. Rotate injection sites between doses.
Reconstitution Steps
- Draw 1.0 mL bacteriostatic water with a sterile syringe
- Inject slowly down the vial wall to avoid foaming
- Gently swirl or roll until dissolved (do not shake)
- Label with date and concentration
- Refrigerate at 2–8 °C (35.6–46.4 °F), protected from light
Result: Approximately 5 mg/mL concentration, allowing 1 insulin unit ≈ 50 mcg on a U-100 syringe
Storage Guidelines
- Lyophilized: −20 °C (−4 °F), dry/dark conditions
- Reconstituted: 2–8 °C (35.6–46.4 °F); do NOT freeze
- Shelf life: 28 days post-reconstitution
Key Administration Notes
- Rotate injection sites to prevent lipohypertrophy
- Generally well tolerated in research; transient injection-site redness is the most commonly noted effect
- Document weekly dose, date, and injection site
- Use sterile syringes; dispose in sharps container
- Allow vials to reach room temperature before opening
⚠️ Research & Educational Purposes Only
This information is provided for research and educational purposes only. Peptides discussed are intended for laboratory research use only and are not approved for human consumption. Always consult qualified healthcare professionals for medical advice. Individual results may vary. This is not medical advice.