AOD-9604 (5 mg Vial) Dosage Protocol

Educational guide for reconstitution and daily dosing of AOD-9604, a C-terminal fragment of hGH studied for fat metabolism and body composition support.

Dosing & Reconstitution Guide

Educational guide for reconstitution and daily dosing

Subcutaneous Protocol (3 mL = 1.667 mg/mL)

Frequency: Inject once daily subcutaneously. AOD-9604 supports fat metabolism with daily administration. Note: A single 5 mg vial provides approximately 10 days of research material at maintenance doses.

Reconstitution Steps

1. Remove the vial from storage and allow to reach room temperature for 10–15 minutes.
2. Draw 3.0 mL bacteriostatic water with a sterile syringe.
3. Inject slowly down the vial wall; avoid foaming.
4. Gently swirl/roll until dissolved—solution should be clear (do not shake).
5. Label and refrigerate at 2–8 °C (35.6–46.4 °F), protected from light; use within 2–4 weeks.

How This Works

AICAR (5-Aminoimidazole-4-carboxamide ribonucleotide) is a synthetic compound that activates AMP-activated protein kinase (AMPK), a key enzyme involved in cellular energy homeostasis. AMPK acts as a metabolic master switch that regulates glucose uptake, fatty acid oxidation, and mitochondrial biogenesis.

By activating AMPK, AICAR may help enhance metabolic efficiency, support endurance performance, and promote fat oxidation. In research settings, AICAR has been studied for its potential to mimic the effects of exercise on cellular metabolism, making it of interest for metabolic and performance research.

5-Amino-1MQ (5-amino-1-methylquinolinium) is a synthetic small molecule that selectively inhibits Nicotinamide N-methyltransferase (NNMT). NNMT is an enzyme that methylates nicotinamide (vitamin B3) using S-adenosylmethionine (SAM) as a methyl donor. In states of obesity and metabolic dysfunction, NNMT is often overexpressed in adipose tissue, depleting nicotinamide and reducing NAD+ availability.

By inhibiting NNMT, 5-Amino-1MQ may spare nicotinamide for NAD+ synthesis, thereby activating SIRT1 (Sirtuin 1) pathways associated with mitochondrial biogenesis and fat oxidation. Preclinical studies indicate that NNMT knockdown or inhibition can protect against diet-induced weight gain and improve metabolic parameters without reducing food intake.

Potential Benefits & Side Effects

Observations from preclinical and early research literature.

• May support reductions in fat mass while preserving lean muscle in animal models
• Associated with elevated NAD+ levels and SIRT1 activation in preclinical studies
• Enhanced grip strength observed in aged mice when combined with exercise
• Generally well tolerated; occasional reports of mild headache, transient jitteriness, or injection-site reactions
• Long-term human safety data not established; this compound remains investigational

Injection Technique

General subcutaneous guidance from clinical best-practice resources.

1. Clean the vial stopper and skin with alcohol; allow to dry.
2. Pinch a skinfold; insert the needle at 45–90° into subcutaneous tissue.
3. Do not aspirate for subcutaneous injections; inject slowly and steadily.
4. Rotate sites systematically (abdomen, thighs, upper arms) to avoid lipohypertrophy.
5. Slow injection may minimize any stinging sensation associated with the compound.

Important Notes

Practical considerations for consistency and safety.

• Use new sterile insulin syringes for each administration; dispose in a sharps container.
• Rotate injection sites (abdomen, thighs, upper arms) to reduce local irritation.
• Inject slowly; a mild stinging sensation may occur due to the quinolinium structure.
• Document daily dose and site rotation to maintain consistency.
• The 10 mg vial format is suited for short-term tolerance assessment; extended protocols require multiple vials.

References