TB-500 (10 mg Vial) Dosage Protocol
Research guide for gradual escalation dosing and reconstitution procedures for TB-500, a synthetic peptide fragment investigated for tissue regeneration and wound healing.
Quickstart Highlights
TB-500 functions as a synthetic peptide fragment corresponding to the active region of thymosin beta‑4. This peptide represents the N-terminal active fragment, specifically the heptapeptide sequence Ac-LKKTETQ, which is responsible for actin-binding and cell-migration properties. Research indicates it promotes angiogenesis, accelerates wound healing, and supports tissue regeneration by upregulating cell motility and blood vessel formation. Important note: TB-500 is not FDA-approved for human use and is for research purposes only.
- Reconstitute: Add 3.0 mL bacteriostatic water → approximately 3.33 mg/mL concentration
- Weekly dosing schedule: 500 mcg → 600 mcg → 750 mcg → 1000 mcg over 12 weeks
- Easy measuring: At 3.33 mg/mL, 1 insulin unit ≈ 33.3 mcg on a U-100 syringe
- Storage: Refrigerate at 2–8 °C (35.6–46.4 °F), protected from light
Dosing & Reconstitution Guide
Standard Daily Titration Protocol with gradual escalation over 8-12 weeks
Daily Subcutaneous Protocol (3 mL = ~3.33 mg/mL)
| Phase | Daily Dose | Units (per injection) | Measurement (mL) |
|---|---|---|---|
| Weeks 1–2 | 500 mcg | 15 units | 0.15 mL |
| Weeks 3–4 | 600 mcg | 18 units | 0.18 mL |
| Weeks 5–8 | 750 mcg | 23 units | 0.23 mL |
| Weeks 9–12 | 1000 mcg | 30 units | 0.30 mL |
Administration: Once daily via subcutaneous injection. The protocol recommends gradual titration over 8-12 weeks, with optional extension to 16 weeks.
Reconstitution Steps
- Draw 3.0 mL bacteriostatic water with a sterile syringe
- Inject slowly down the vial wall to avoid foaming
- Gently swirl or roll until dissolved (do not shake)
- Label with date and concentration
- Refrigerate at 2–8 °C (35.6–46.4 °F), protected from light
Result: Approximately 3.33 mg/mL concentration, allowing 1 insulin unit ≈ 33.3 mcg on a U-100 syringe
Storage Guidelines
- Lyophilized: −20 °C (−4 °F), dry/dark conditions
- Reconstituted: 2–8 °C (35.6–46.4 °F); do NOT freeze
- Shelf life: 28 days post-reconstitution
Key Administration Notes
- Rotate injection sites to prevent lipohypertrophy
- Gastrointestinal effects common initially; titration minimizes them
- Document weekly dose, date, and injection site
- Use sterile syringes; dispose in sharps container
- Allow vials to reach room temperature before opening
⚠️ Research & Educational Purposes Only
This information is provided for research and educational purposes only. Peptides discussed are intended for laboratory research use only and are not approved for human consumption. Always consult qualified healthcare professionals for medical advice. Individual results may vary. This is not medical advice.