Semaglutide (20 mg Vial) Dosage Protocol
Research guide for gradual escalation dosing and reconstitution procedures for Semaglutide, a GLP-1 receptor agonist investigated for metabolic regulation and chronic weight management.
Quickstart Highlights
Semaglutide operates as a GLP-1 receptor agonist featuring an extended half-life of approximately 7 days, permitting convenient once-weekly dosing. This compound stimulates appetite reduction and improves metabolic markers through central nervous system pathways and enhanced insulin secretion mechanisms.
- Reconstitute: Add 3.0 mL bacteriostatic water → ~6.67 mg/mL concentration
- Weekly dosing schedule: 0.25 mg → 0.5 mg → 1.0 mg → 1.7 mg → 2.4 mg over 17+ weeks
- Easy measuring: At 6.67 mg/mL, 1 U-100 unit ≈ 66.7 mcg
- Storage: Reconstituted solution at 2–8°C, protected from light, use within 28 days
Dosing & Reconstitution Guide
Standard Gradual Titration for weekly subcutaneous administration
Weekly Subcutaneous Protocol (3 mL = ~6.67 mg/mL)
| Weeks | Weekly Dose | Units (U-100 Syringe) | Volume (mL) |
|---|---|---|---|
| 1–4 | 250 mcg (0.25 mg) | 4 units | 0.04 mL |
| 5–8 | 500 mcg (0.5 mg) | 7.5 units | 0.075 mL |
| 9–12 | 1000 mcg (1.0 mg) | 15 units | 0.15 mL |
| 13–16 | 1700 mcg (1.7 mg) | 25.5 units | 0.255 mL |
| 17+ | 2400 mcg (2.4 mg) | 36 units | 0.36 mL |
Administration: Once weekly via subcutaneous injection, same day each week.
Reconstitution Steps
- Draw 3.0 mL bacteriostatic water using sterile syringe
- Inject slowly along vial wall to minimize foaming; avoid shaking
- Swirl or roll vial gently until powder fully dissolves
- Label with reconstitution date; refrigerate at 2–8°C, protected from light
- Use within 28 days for maximum potency and safety
Result: ~6.67 mg/mL concentration; 1 U-100 unit ≈ 66.7 mcg
Storage Guidelines
- Lyophilized: −20 °C (−4 °F), dry/dark conditions
- Reconstituted: 2–8 °C (35.6–46.4 °F); do NOT freeze
- Shelf life: 28 days post-reconstitution
Key Administration Notes
- Rotate injection sites to prevent lipohypertrophy
- Gastrointestinal effects common initially; titration minimizes them
- Document weekly dose, date, and injection site
- Use sterile syringes; dispose in sharps container
- Allow vials to reach room temperature before opening
⚠️ Research & Educational Purposes Only
This information is provided for research and educational purposes only. Peptides discussed are intended for laboratory research use only and are not approved for human consumption. Always consult qualified healthcare professionals for medical advice. Individual results may vary. This is not medical advice.