L-Carnitine 200mg Dosage Protocol

Educational guide for reconstitution and administration of L-Carnitine, a nutrient studied for energy metabolism and mitochondrial function.

Dosing & Reconstitution Guide

Educational guide for reconstitution and daily dosing

Subcutaneous Protocol (2 mL = 5 mg/mL)

Frequency: Inject once or twice daily subcutaneously. Due to the compound's plasma half-life of approximately 3.8–6.9 hours, twice-daily (BID) dosing may provide more sustained NNMT inhibition. Note: A single 10 mg vial provides only 2–4 days of research material at these doses.

Reconstitution Steps

1. Remove the vial from freezer storage (−20 °C / −4 °F) and allow to equilibrate at room temperature for 15–20 minutes.
2. Draw 2.0 mL bacteriostatic water with a sterile syringe.
3. Inject slowly down the vial wall; avoid foaming.
4. Gently swirl/roll until dissolved—solution should be clear (do not shake).
5. Label and refrigerate at 2–8 °C (35.6–46.4 °F), protected from light; use within 2–4 weeks.

Biological Action

L-Carnitine represents an essential nutrient and quaternary ammonium compound that plays a critical role in mitochondrial fatty acid oxidation and energy metabolism.

As a carrier molecule, L-carnitine facilitates the transport of long-chain fatty acids across mitochondrial membranes for beta-oxidation and ATP production. It serves as an essential cofactor in cellular energy metabolism.

L-Carnitine has been extensively studied for its role in supporting mitochondrial function and energy production in research contexts.

Potential Benefits & Side Effects

Findings from metabolic and mitochondrial research.

• Potential to support mitochondrial function and energy metabolism in research settings
• May assist with fatty acid oxidation and cellular energy studies
• Associated with metabolic regulation and mitochondrial support
• Generally well tolerated; occasional mild gastrointestinal effects
• Human safety profile established; dietary supplement
• Long-term human safety data not established; this compound remains investigational

Injection Technique

General subcutaneous guidance from clinical best-practice resources.

1. Clean the vial stopper and skin with alcohol; allow to dry.
2. Pinch a skinfold; insert the needle at 45–90° into subcutaneous tissue.
3. Do not aspirate for subcutaneous injections; inject slowly and steadily.
4. Rotate sites systematically (abdomen, thighs, upper arms) to avoid lipohypertrophy.
5. Slow injection may minimize any stinging sensation associated with the compound.

Important Notes

Practical considerations for consistency and safety.

• Use new sterile insulin syringes for each administration; dispose in a sharps container.
• Rotate injection sites (abdomen, thighs, upper arms) to reduce local irritation.
• Inject slowly; a mild stinging sensation may occur due to the quinolinium structure.
• Document daily dose and site rotation to maintain consistency.
• The 10 mg vial format is suited for short-term tolerance assessment; extended protocols require multiple vials.

References