SS-31 (10 mg Vial) Dosage Protocol
Reconstitution and dosing guide for the 10 mg SS-31 (elamipretide) vial — a mitochondria-targeting peptide studied for cellular energy and oxidative protection.
Quickstart Highlights
SS-31 (elamipretide) is a cell-permeable, mitochondria-targeting tetrapeptide that concentrates on the inner mitochondrial membrane and binds cardiolipin. Research studies it for stabilizing mitochondrial structure (cristae), improving electron transport and ATP production, and reducing reactive oxygen species. It is investigated for mitochondrial, cardiac, muscular, and age-related conditions, and is administered subcutaneously.
- Composition: 10 mg SS-31 (elamipretide) per vial
- Reconstitute: Add 1.0 mL bacteriostatic water → 10 mg/mL concentration
- Easy measuring: At 10 mg/mL, 1 unit (0.01 mL) on a U-100 syringe = 100 mcg (so 5 mg = 50 units)
- Storage: Lyophilized: −20 °C (−4 °F); reconstituted: 2–8 °C (35.6–46.4 °F), protected from light, use within 2–4 weeks
Dosing & Reconstitution Guide
Once-daily subcutaneous protocol; doses are in milligrams
Daily Subcutaneous Protocol (10 mg vial, 1.0 mL = 10 mg/mL)
| Protocol | Daily Dose | Units (U-100) | mL |
|---|---|---|---|
| Standard | 5 mg once daily | 50 units | 0.50 mL |
| Lower | 2.5 mg once daily | 25 units | 0.25 mL |
Administration: Inject once daily subcutaneously, rotating sites. At 10 mg/mL each milligram equals 10 units (0.10 mL). A 10 mg vial provides roughly 2 days at the 5 mg standard dose.
Reconstitution Steps
- Allow the vial to reach room temperature (15–20 minutes out of cold storage).
- Draw 1.0 mL bacteriostatic water with a sterile syringe.
- Inject slowly down the vial wall to avoid foaming.
- Gently swirl or roll until fully dissolved (do not shake).
- Label with date and concentration; refrigerate at 2–8 °C, protected from light.
Result: 10 mg/mL concentration
How This Works
SS-31 selectively associates with cardiolipin, a phospholipid unique to the inner mitochondrial membrane. By protecting cardiolipin and preserving cristae architecture, it supports efficient electron transport and ATP synthesis while limiting the leakage of reactive oxygen species.
Unlike general antioxidants, it acts at the source of mitochondrial energy production, which is why research has focused on tissues with high energy demand such as heart and skeletal muscle.
Key Administration Notes
- Inject once daily subcutaneously; rotate sites.
- Use new sterile insulin syringes; dispose in a sharps container.
- Reconstituting all three vial sizes to 10 mg/mL keeps measuring identical across them.
- Document dose, timing, and site for consistency.
References
Szeto HH. First-in-class cardiolipin-protective compound (SS-31/elamipretide) as a therapeutic agent. View Source
Szeto HH, et al. Mitochondria-targeted peptide protects against injury. View Source
⚠️ Research & Educational Purposes Only
This information is provided for research and educational purposes only. The compounds discussed are intended for laboratory research use only and are not approved for human consumption. Always consult qualified healthcare professionals for medical advice. Individual results may vary. This is not medical advice.