Semaglutide (10 mg Vial) Dosage Protocol
Research guide for gradual escalation dosing and reconstitution procedures for Semaglutide, a GLP-1 receptor agonist investigated for metabolic regulation and chronic weight management.
Quickstart Highlights
Semaglutide functions as a GLP-1 receptor agonist authorized for chronic weight management and glycemic control. With an extended half-life of approximately 7 days, this medication allows for convenient once-weekly dosing. The compound operates through multiple mechanisms including diminished appetite via central nervous system pathways, delayed gastric emptying, and glucose-dependent insulin secretion stimulation.
- Reconstitute: Add 3.0 mL bacteriostatic water → ~3.33 mg/mL concentration
- Weekly dosing schedule: 0.25 mg → 0.5 mg → 1.0 mg → 1.7 mg → 2.4 mg over 17+ weeks
- Easy measuring: At 3.33 mg/mL, 1 unit ≈ 33.3 mcg on a U-100 insulin syringe
- Storage: Reconstituted solution at 2–8 °C (35.6–46.4 °F), protected from light, use within 28 days
Dosing & Reconstitution Guide
Standard Gradual Titration for weekly subcutaneous administration
Weekly Subcutaneous Protocol (3 mL = ~3.33 mg/mL)
| Week Range | Weekly Dose | Units (U-100 Syringe) | Volume (mL) |
|---|---|---|---|
| 1–4 | 250 mcg (0.25 mg) | 7.5 units | 0.075 mL |
| 5–8 | 500 mcg (0.5 mg) | 15 units | 0.15 mL |
| 9–12 | 1000 mcg (1.0 mg) | 30 units | 0.30 mL |
| 13–16 | 1700 mcg (1.7 mg) | 51 units | 0.51 mL |
| 17+ (Maintenance) | 2400 mcg (2.4 mg) | 72 units | 0.72 mL |
Frequency: One subcutaneous injection weekly on the same day each week, with or without meals.
Reconstitution Steps
- Draw 3.0 mL bacteriostatic water using a sterile syringe
- Inject slowly down the vial wall to minimize foaming; avoid shaking
- Gently swirl or roll until powder fully dissolves
- Label with reconstitution date; refrigerate at 2–8 °C (35.6–46.4 °F), protected from light
- Use within 28 days for maximum potency and safety
Result: ~3.33 mg/mL concentration; 1 unit on U-100 syringe ≈ 33.3 mcg
Storage Guidelines
- Reconstituted: 2–8 °C (35.6–46.4 °F), protected from light
- Shelf life: 28 days post-reconstitution
Key Administration Notes
- Rotate injection sites to prevent lipohypertrophy
- Gastrointestinal effects common initially; titration minimizes them
- Document weekly dose, date, and injection site
- Use sterile syringes; dispose in sharps container
- Allow vials to reach room temperature before opening
⚠️ Research & Educational Purposes Only
This information is provided for research and educational purposes only. Peptides discussed are intended for laboratory research use only and are not approved for human consumption. Always consult qualified healthcare professionals for medical advice. Individual results may vary. This is not medical advice.