Glutathione (600 mg Vial) Dosage Protocol
Reconstitution and dosing guide for the 600 mg Glutathione vial — the body's master antioxidant tripeptide, studied for oxidative stress, detoxification support, and skin health.
Quickstart Highlights
Glutathione (GSH) is a tripeptide of glutamate, cysteine, and glycine — the body's principal intracellular antioxidant. Research and wellness use focuses on free-radical scavenging, support of liver detoxification (phase-II conjugation), immune function, and skin brightening. It is commonly administered by subcutaneous or intramuscular injection (and clinically by IV).
- Composition: 600 mg Glutathione per vial
- Reconstitute: Add 3.0 mL bacteriostatic water → 200 mg/mL concentration
- Easy measuring: At 200 mg/mL, 1 unit (0.01 mL) = 2 mg; 0.1 mL (10 units) = 20 mg
- Storage: Lyophilized: −20 °C (−4 °F); reconstituted: 2–8 °C (35.6–46.4 °F), protected from light, use within 2–4 weeks
Dosing & Reconstitution Guide
Subcutaneous or intramuscular protocol, typically a few times per week
Protocol (600 mg vial, 3 mL = 200 mg/mL)
| Protocol | Per-dose | Frequency | Volume |
|---|---|---|---|
| Standard | 200 mg | 2–3× per week | 1.0 mL (100 units) |
| Higher | 400 mg | 1–2× per week | 2.0 mL (split sites) |
Administration: Inject subcutaneously or intramuscularly. Because doses are in the hundreds of milligrams, injection volumes are larger than typical peptides — split volumes over 1.0 mL across two sites. Glutathione solutions should be clear; discard if discolored.
Reconstitution Steps
- Allow the vial to reach room temperature (15–20 minutes out of cold storage).
- Draw 3.0 mL bacteriostatic water with a sterile syringe.
- Inject slowly down the vial wall to avoid foaming.
- Gently swirl or roll until fully dissolved (do not shake).
- Label with date and concentration; refrigerate at 2–8 °C, protected from light.
Result: 200 mg/mL concentration
How This Works
Glutathione neutralizes reactive oxygen species directly and serves as a cofactor for antioxidant enzymes (glutathione peroxidases). It also conjugates toxins and drug metabolites in the liver, making them water-soluble for excretion.
Its popularity in skin protocols stems from inhibition of tyrosinase (a key enzyme in melanin production), which research associates with a lighter, more even skin tone over time.
Key Administration Notes
- Doses are in milligrams; injection volumes are larger — split anything over 1.0 mL.
- Use new sterile syringes; rotate sites; dispose in a sharps container.
- Discard if the solution becomes discolored or cloudy.
- Document dose, route, and frequency for consistency.
References
Pizzorno J. Glutathione! View Source
Wu G, et al. Glutathione metabolism and its implications for health. View Source
⚠️ Research & Educational Purposes Only
This information is provided for research and educational purposes only. The compounds discussed are intended for laboratory research use only and are not approved for human consumption. Always consult qualified healthcare professionals for medical advice. Individual results may vary. This is not medical advice.