NAD+ 500mg Dosage Protocol

Educational guide for reconstitution and administration of NAD+, a coenzyme studied for cellular energy metabolism and redox balance.

Dosing & Reconstitution Guide

Educational guide for reconstitution and daily dosing

Subcutaneous Protocol (2 mL = 5 mg/mL)

Frequency: Inject once or twice daily subcutaneously. Due to the compound's plasma half-life of approximately 3.8–6.9 hours, twice-daily (BID) dosing may provide more sustained NNMT inhibition. Note: A single 10 mg vial provides only 2–4 days of research material at these doses.

Reconstitution Steps

1. Remove the vial from freezer storage (−20 °C / −4 °F) and allow to equilibrate at room temperature for 15–20 minutes.
2. Draw 2.0 mL bacteriostatic water with a sterile syringe.
3. Inject slowly down the vial wall; avoid foaming.
4. Gently swirl/roll until dissolved—solution should be clear (do not shake).
5. Label and refrigerate at 2–8 °C (35.6–46.4 °F), protected from light; use within 2–4 weeks.

Biological Action

NAD+ (Nicotinamide Adenine Dinucleotide) represents an essential coenzyme that functions as a critical electron carrier in cellular redox reactions and energy metabolism.

As a redox cofactor, NAD+ participates in numerous enzymatic reactions including glycolysis, the citric acid cycle, and oxidative phosphorylation, serving as a substrate for sirtuins and poly(ADP-ribose) polymerases.

NAD+ has been studied extensively for its role in cellular energy metabolism and potential applications in age-related metabolic disorders.

Potential Benefits & Side Effects

Findings from redox biology and cellular metabolism research.

• Potential to support cellular energy metabolism and redox balance in research settings
• May assist with mitochondrial function and sirtuin activation studies
• Associated with cellular metabolism and age-related metabolic studies
• Generally well tolerated; occasional mild flushing or gastrointestinal effects
• Human safety profile established; dietary supplement
• Long-term human safety data not established; this compound remains investigational

Injection Technique

General subcutaneous guidance from clinical best-practice resources.

1. Clean the vial stopper and skin with alcohol; allow to dry.
2. Pinch a skinfold; insert the needle at 45–90° into subcutaneous tissue.
3. Do not aspirate for subcutaneous injections; inject slowly and steadily.
4. Rotate sites systematically (abdomen, thighs, upper arms) to avoid lipohypertrophy.
5. Slow injection may minimize any stinging sensation associated with the compound.

Important Notes

Practical considerations for consistency and safety.

• Use new sterile insulin syringes for each administration; dispose in a sharps container.
• Rotate injection sites (abdomen, thighs, upper arms) to reduce local irritation.
• Inject slowly; a mild stinging sensation may occur due to the quinolinium structure.
• Document daily dose and site rotation to maintain consistency.
• The 10 mg vial format is suited for short-term tolerance assessment; extended protocols require multiple vials.

References