Livagen 20mg Dosage Protocol
Educational guide for reconstitution and administration of Livagen, a peptide studied for tissue repair and organ function support.
Quickstart Highlights
Livagen dosage protocols focus on this synthetic peptide derived from pro-collagen type III alpha-1 C-terminal fragment, studied for its effects on tissue repair and organ function.
- Reconstitute: Add 3.0 mL bacteriostatic water → ~6.67 mg/mL concentration
- Typical protocols: 100–300 mcg administered 1-2 times daily (subcutaneous)
- Easy measuring: At 6.67 mg/mL, 1 unit = 0.01 mL ≈ 66.7 mcg on a U-100 insulin syringe
- Storage: Lyophilized: freeze at −20 °C (−4 °F); after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F) and use within 2–4 weeks
Dosing & Reconstitution Guide
Educational guide for reconstitution and daily dosing
Subcutaneous Protocol (2 mL = 5 mg/mL)
| Phase | Daily Dose (mg) | Units (per injection) (mL) |
|---|---|---|
| Weeks 1–2 | 0.5 mg once daily | 8 units (0.08 mL) |
| Weeks 3–4 | 1.0 mg once daily | 15 units (0.15 mL) |
| Weeks 5–8+ | 2.0 mg once daily | 30 units (0.30 mL) |
Frequency: Inject once or twice daily subcutaneously. Due to the compound's plasma half-life of approximately 3.8–6.9 hours, twice-daily (BID) dosing may provide more sustained NNMT inhibition. Note: A single 10 mg vial provides only 2–4 days of research material at these doses.
Reconstitution Steps
- Remove the vial from freezer storage (−20 °C / −4 °F) and allow to equilibrate at room temperature for 15–20 minutes.
- Draw 3.0 mL bacteriostatic water with a sterile syringe.
- Inject slowly down the vial wall; avoid foaming.
- Gently swirl/roll until dissolved—solution should be clear (do not shake).
- Label and refrigerate at 2–8 °C (35.6–46.4 °F), protected from light; use within 2–4 weeks.
Biological Action
Livagen represents a synthetic peptide derived from the C-terminal fragment of pro-collagen type III alpha-1, studied for its effects on tissue repair and organ function support.
As a collagen-derived peptide fragment, Livagen has been investigated for its potential to support extracellular matrix integrity and tissue homeostasis.
Livagen has been studied for its role in supporting connective tissue function and cellular matrix maintenance in research contexts.
Potential Benefits & Side Effects
Findings from tissue repair and connective tissue research.
- Potential to support tissue repair and extracellular matrix integrity in research settings
- May assist with connective tissue function and cellular matrix maintenance
- Associated with collagen support and tissue homeostasis studies
- Generally well tolerated; occasional mild injection reactions
- Human safety profile remains under investigation; not approved for therapeutic use
- Long-term human safety data not established; this compound remains investigational
Injection Technique
General subcutaneous guidance from clinical best-practice resources.
- Clean the vial stopper and skin with alcohol; allow to dry.
- Pinch a skinfold; insert the needle at 45–90° into subcutaneous tissue.
- Do not aspirate for subcutaneous injections; inject slowly and steadily.
- Rotate sites systematically (abdomen, thighs, upper arms) to avoid lipohypertrophy.
- Slow injection may minimize any stinging sensation associated with the compound.
Important Notes
Practical considerations for consistency and safety.
- Use new sterile insulin syringes for each administration; dispose in a sharps container.
- Rotate injection sites (abdomen, thighs, upper arms) to reduce local irritation.
- Inject slowly; a mild stinging sensation may occur due to the quinolinium structure.
- Document daily dose and site rotation to maintain consistency.
- The 10 mg vial format is suited for short-term tolerance assessment; extended protocols require multiple vials.
Important Disclaimer: This content is intended for educational purposes only and does not constitute medical advice, diagnosis, or treatment. This is not medical advice and is for informational purposes only. Always consult with a qualified healthcare professional before starting any new research protocol.
References
Nicotinamide N-methyltransferase knockdown protects against diet-induced obesity View Source
Nicotinamide N-methyltransferase inhibition mitigates obesity-related metabolic dysfunctions View Source
NNMT: a novel therapeutic target for metabolic syndrome View Source