Livagen 20mg Dosage Protocol

Educational guide for reconstitution and administration of Livagen, a peptide studied for tissue repair and organ function support.

Quickstart Highlights

Livagen dosage protocols focus on this synthetic peptide derived from pro-collagen type III alpha-1 C-terminal fragment, studied for its effects on tissue repair and organ function.

  • Reconstitute: Add 3.0 mL bacteriostatic water → ~6.67 mg/mL concentration
  • Typical protocols: 100–300 mcg administered 1-2 times daily (subcutaneous)
  • Easy measuring: At 6.67 mg/mL, 1 unit = 0.01 mL ≈ 66.7 mcg on a U-100 insulin syringe
  • Storage: Lyophilized: freeze at −20 °C (−4 °F); after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F) and use within 2–4 weeks

Dosing & Reconstitution Guide

Educational guide for reconstitution and daily dosing

Subcutaneous Protocol (2 mL = 5 mg/mL)

Phase Daily Dose (mg) Units (per injection) (mL)
Weeks 1–2 0.5 mg once daily 8 units (0.08 mL)
Weeks 3–4 1.0 mg once daily 15 units (0.15 mL)
Weeks 5–8+ 2.0 mg once daily 30 units (0.30 mL)

Frequency: Inject once or twice daily subcutaneously. Due to the compound's plasma half-life of approximately 3.8–6.9 hours, twice-daily (BID) dosing may provide more sustained NNMT inhibition. Note: A single 10 mg vial provides only 2–4 days of research material at these doses.

Reconstitution Steps

  1. Remove the vial from freezer storage (−20 °C / −4 °F) and allow to equilibrate at room temperature for 15–20 minutes.
  2. Draw 3.0 mL bacteriostatic water with a sterile syringe.
  3. Inject slowly down the vial wall; avoid foaming.
  4. Gently swirl/roll until dissolved—solution should be clear (do not shake).
  5. Label and refrigerate at 2–8 °C (35.6–46.4 °F), protected from light; use within 2–4 weeks.

Biological Action

Livagen represents a synthetic peptide derived from the C-terminal fragment of pro-collagen type III alpha-1, studied for its effects on tissue repair and organ function support.

As a collagen-derived peptide fragment, Livagen has been investigated for its potential to support extracellular matrix integrity and tissue homeostasis.

Livagen has been studied for its role in supporting connective tissue function and cellular matrix maintenance in research contexts.

Potential Benefits & Side Effects

Findings from tissue repair and connective tissue research.

Injection Technique

General subcutaneous guidance from clinical best-practice resources.

  1. Clean the vial stopper and skin with alcohol; allow to dry.
  2. Pinch a skinfold; insert the needle at 45–90° into subcutaneous tissue.
  3. Do not aspirate for subcutaneous injections; inject slowly and steadily.
  4. Rotate sites systematically (abdomen, thighs, upper arms) to avoid lipohypertrophy.
  5. Slow injection may minimize any stinging sensation associated with the compound.

Important Notes

Practical considerations for consistency and safety.

Important Disclaimer: This content is intended for educational purposes only and does not constitute medical advice, diagnosis, or treatment. This is not medical advice and is for informational purposes only. Always consult with a qualified healthcare professional before starting any new research protocol.

References

Nature Medicine (2014)
Nicotinamide N-methyltransferase knockdown protects against diet-induced obesity View Source
PMC (2024)
Nicotinamide N-methyltransferase inhibition mitigates obesity-related metabolic dysfunctions View Source
Frontiers in Pharmacology (2024)
NNMT: a novel therapeutic target for metabolic syndrome View Source