Pinealon 20mg Dosage Protocol

Educational guide for reconstitution and administration of Pinealon, a peptide bioregulator studied for brain function and circadian rhythms.

Dosing & Reconstitution Guide

Educational guide for reconstitution and daily dosing

Subcutaneous Protocol (2 mL = 5 mg/mL)

Frequency: Inject once or twice daily subcutaneously. Due to the compound's plasma half-life of approximately 3.8–6.9 hours, twice-daily (BID) dosing may provide more sustained NNMT inhibition. Note: A single 10 mg vial provides only 2–4 days of research material at these doses.

Reconstitution Steps

1. Remove the vial from freezer storage (−20 °C / −4 °F) and allow to equilibrate at room temperature for 15–20 minutes.
2. Draw 2.0 mL bacteriostatic water with a sterile syringe.
3. Inject slowly down the vial wall; avoid foaming.
4. Gently swirl/roll until dissolved—solution should be clear (do not shake).
5. Label and refrigerate at 2–8 °C (35.6–46.4 °F), protected from light; use within 2–4 weeks.

Biological Action

Pinealon represents a peptide bioregulator derived from pineal gland tissue extracts, studied for its effects on brain function and circadian rhythm regulation.

As a pineal gland-derived peptide complex, Pinealon has been investigated for its potential to support melatonin production and regulate circadian rhythms.

Pinealon has been studied for its role in supporting natural brain function and sleep-wake cycle regulation in research contexts.

Potential Benefits & Side Effects

Findings from neuroendocrinology and sleep research.

• Potential to support brain function and circadian rhythm regulation in research settings
• May assist with melatonin production and sleep quality studies
• Associated with pineal gland function and neuroprotection
• Generally well tolerated; occasional mild drowsiness or local reactions
• Human safety profile remains under investigation; not approved for therapeutic use
• Long-term human safety data not established; this compound remains investigational

Injection Technique

General subcutaneous guidance from clinical best-practice resources.

1. Clean the vial stopper and skin with alcohol; allow to dry.
2. Pinch a skinfold; insert the needle at 45–90° into subcutaneous tissue.
3. Do not aspirate for subcutaneous injections; inject slowly and steadily.
4. Rotate sites systematically (abdomen, thighs, upper arms) to avoid lipohypertrophy.
5. Slow injection may minimize any stinging sensation associated with the compound.

Important Notes

Practical considerations for consistency and safety.

• Use new sterile insulin syringes for each administration; dispose in a sharps container.
• Rotate injection sites (abdomen, thighs, upper arms) to reduce local irritation.
• Inject slowly; a mild stinging sensation may occur due to the quinolinium structure.
• Document daily dose and site rotation to maintain consistency.
• The 10 mg vial format is suited for short-term tolerance assessment; extended protocols require multiple vials.

References